The localization of partial discharge (PD) sources is of importance for the monitoring and maintenance of power transformers. Time difference of arrival (TDoA) based methods are widely adopted in the literature for the localization of PDs. Recently, time reversal (TR) was suggested as an efficient means to locate PD sources. As opposed to TDoA, which needs at least 4 sensors, TR is able to locate PD sources in power transformers with only one sensor. Moreover, it needs neither line-of-sight wave propagation from the PD sources to the sensor nor time synchronization. In this study, we present for the first time an experimental demonstration of the ability of the TR process to locate PD sources. A typical TR process includes three steps: (1) recording the PD-emitted field by a sensor, (2) time reversing and back injecting the signal into the medium, (3) using a proper criterion to obtain the focusing point which corresponds to the location of the PD source. In this work, we present a laboratory setup in which steps one and two are performed experimentally, both in the frequency and in the time domain. The obtained peak electric field value is used as a criterion in the third step. It is found that the accuracy of the proposed method is better than 2.5 cm in a transformer tank model with dimensions 73 × 73 × 103 cm3. The effects of the presence of scatterers such as transformer windings are also investigated experimentally and found not to affect the location accuracy of the method.