NIR-emitting and photo-thermal active nanogold as mitochondria-specific probes
Chakrabortty, Sabyasachi; Sison, Miguel; Wu, Yuzhou; Ladenburger, Anita; Pramanik, Goutam; Biskupek, Johannes; Extermann, Jérôme; Kaiser, Ute; Lasser, Theo; Weil, Tanja
2017

Résumé

We report a bioinspired multifunctional albumin derived polypeptide coating comprising grafted poly(ethylene oxide) chains, multiple copies of the HIV TAT derived peptide enabling cellular uptake as well as mitochondria targeting triphenyl-phosphonium (TPP) groups. Exploring these polypeptide copolymers for passivating gold nanoparticles (Au NPs) yielded (i) NIR-emitting markers in confocal microscopy and (ii) photo-thermal active probes in optical coherence microscopy. We demonstrate the great potential of such multifunctional protein-derived biopolymer coatings for efficiently directing Au NP into cells and to subcellular targets to ultimately probe important cellular processes such as mitochondria dynamics and vitality inside living cells.

Détails

Titre NIR-emitting and photo-thermal active nanogold as mitochondria-specific probes
Auteur(s)/ trice(s) Chakrabortty, Sabyasachi (Insitute of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany)
Sison, Miguel (EPFL, Lausanne, Switzerland)
Wu, Yuzhou (Insitute of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany)
Ladenburger, Anita (Central Facility of Electron Microscopy, University of Ulm, Ulm, Germany)
Pramanik, Goutam (Insitute of Organic Chemistry III, Ulm University, Ulm, Germany)
Biskupek, Johannes (Central Facility of Electron Microscopy, University of Ulm, Ulm, Germany)
Extermann, Jérôme (EPFL, Lausanne, Switzerland ; School of Engineering, Architecture and Landscape (hepia), HES-SO University of Applied Sciences Western Switzerland)
Kaiser, Ute (Central Facility of Electron Microscopy, University of Ulm, Ulm, Germany)
Lasser, Theo (EPFL, Lausanne, Switzerland)
Weil, Tanja (Institute of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany)
Date 2017-02
Publié dans Biomaterials Science
Volume 2017, vol. 5, no. 5, article no. 966-971
Pagination 10 p.
DOI https://doi.org/10.1039/C6BM00951D
ISSN 2047-4830
Type d'article scientifique
Domaine Ingénierie et Architecture
Ecole HEPIA - Genève
Institut inSTI - Institut des Sciences et Technologies industrielles
Le document apparaît dans Articles scientifiques
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