Non-destructive direct measurements of mechanical properties of biological tissue, such as that of the crystalline lens of the eye are difficult to obtain. More often indirect measurement methods are used, which then require post processing of the data to extract meaningful results which can be translated into material properties, such as the shear modulus of elasticity of the crystalline lens. This paper will present how COMSOL® can be used as a tool for the processing step of deducing the shear modulus of the crystalline lens. Moreover, we will discuss the use of the COMSOL® Optimization module to reverse engineer the material property; as well as the use of COMSOL’s® Application Builder to create a Graphical User Interface (GUI) which enables non-simulation experts to use the simulation model.