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Continuous micro-production using enzymatic reaction and online monitoring

Moser, Regula; Chappuis, Thierry; Vanoli, Ennio; Crelier, Simon; Naef, Olivier
2010
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American Psychological Association 7th edition (APA 7th)
🇺🇸 English, US
Moser, R., Chappuis, T., Vanoli, E., Crelier, S., & Naef, O. (2010). Continuous Micro-Production Using Enzymatic Reaction and Online Monitoring. CHIMIA, 64(11), 799. https://doi.org/10.2533/chimia.2010.799
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Résumé

A micro-reactor coupled to a microfluidic system and an online UV/VIS spectrometer is described. The enzymatic reaction studied is the hydrolysis of the N-benzoyl-L-tyrosine ethyl ester (BTEE) to N-benzoyl-L-tyrosine (BT) and ethanol, catalyzed by chymotrypsin. The production is online monitored with UV spectroscopy at 256 nm. Three different immobilization methods of the enzyme are discussed: Eupergit® C, controlled-pore glass (CPG), and Sepharose.

Détails

Titre
Continuous micro-production using enzymatic reaction and online monitoring
Auteur(s)/ trice(s)
Moser, Regula (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Chappuis, Thierry (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Vanoli, Ennio (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Crelier, Simon (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Naef, Olivier (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Date
2010-11
Publié dans
CHIMIA International Journal for Chemistry
Volume
2010, vol. 64, no. 11, pp. 799-802
Pagination & équivalents
4 p.
DOI
https://doi.org/10.2533/chimia.2010.799
ISSN
0009-4293
Mots-clés (libres)
chymotrypsin ; enzymatic reaction ; enzyme immobilization ; micro-production ; micro-reactor
Type d'article
scientifique
Domaine
Ingénierie et Architecture
Ecole
HEIA-FR
Institut
ChemTech - Institut des technologies chimiques
Le document apparaît dans
Articles scientifiques
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