This chapter clarifies the important aspects of biomedical texture analysis under the general framework introduced in Chapter 1. It was proposed that any approach can be character-ized as the combination of local texture operators and regional aggregation functions. The type of scale and directional information that can or cannot be modeled by categories of texture processing methods is revealed through theoretic analyses and experimental valida-tions. Several key aspects are found to be commonly overlooked in the literature and are highlighted. First, we demonstrate the risk of using regions of interest for aggregation that are regrouping tissue types of different natures. Second, a detailed study of the type of directional information important for biomedical texture characterization suggests that fun-damental properties lie in the local organization of image directions. In addition, it was found that most approaches cannot efficiently characterize the latter, and even fewer can do it with invariance to local rotations. We conclude by deriving novel comparison axes to evalu-ate the relevance of biomedical texture analysis methods in a specific medical or biological applicative context.