000015258 001__ 15258
000015258 005__ 20250107133956.0
000015258 022__ $$a2304-8158
000015258 0247_ $$2DOI$$a10.3390/foods13203251
000015258 037__ $$aARTICLE
000015258 039_9 $$a2025-01-07 13:39:56$$b0$$c2025-01-07 10:39:12$$d1001044$$c2025-01-06 16:04:35$$d0$$y2025-01-06 16:04:27$$z1000099
000015258 041__ $$aeng
000015258 245__ $$aSublethal damage caused by cold plasma on bacillus cereus cells :$$bimpact on cell viability and biofilm-forming capacity
000015258 260__ $$aBasel, Switzerland$$bMDPI
000015258 269__ $$a2024-10
000015258 300__ $$a17 p.
000015258 506__ $$avisible
000015258 520__ $$9eng$$aThe Bacillus cereus group represents a serious risk in powdered and amylaceous foodstuffs. Cold plasma (the fourth state of matter) is emerging as an alternative effective nonthermal technology for pasteurizing a wide range of matrices in solid, liquid, and powder form. The present study aims to evaluate the mechanisms involved in Bacillus cereus inactivation via cold plasma, focusing on (i) the technology’s ability to generate damage in cells (at the morphological and molecular levels) and (ii) studying the effectiveness of cold plasma in biofilm mitigation through the direct effect and inhibition of the biofilm-forming capacity of sublethally damaged cells post-treatment. Dielectric barrier discharge cold plasma (DBD-CP) technology was used to inactivate B. cereus, B. thuringiensis, and B. mycoides under plasma power settings of 100, 200, and 300 W and treatment times ranging from 1 to 10 min. Inactivation levels were achieved in 2–7 log10 cycles under the studied conditions. Percentages of sublethally damaged cells were observed in a range of 45–98%, specifically at treatment times below 7 min. The sublethally damaged cells showed poration, erosion, and loss of integrity at the superficial level. At the molecular level, proteins and DNA leakage were also observed for B. cereus but were minimal for B. mycoides. Biofilms formed by B. cereus were progressively disintegrated under the DBD-CP treatment. The greater the CP treatment intensity, the greater the tearing of the bacteria’s biofilm network. Additionally, cells sublethally damaged by DBD-CP were evaluated in terms of their biofilm-forming capacity. Significant losses in the damaged cells’ biofilm network density and aggregation capacity were observed when B. cereus was recovered after inactivation at 300 W for 7.5 min, compared with the untreated cells. These results provide new insights into the future of tailored DBD-CP design conditions for both the inactivation and biofilm reduction capacity of B. cereus sensu lato species, demonstrating the effectiveness of cold plasma and the risks associated with sublethal damage generation.
000015258 540__ $$acorrect
000015258 592__ $$aHEI-VS
000015258 592__ $$bInstitut Sciences du vivant
000015258 592__ $$cIngénierie et Architecture
000015258 6531_ $$9eng$$asublethal damage
000015258 6531_ $$9eng$$acold plasma
000015258 6531_ $$9eng$$abacillus cereus
000015258 6531_ $$9eng$$aviability
000015258 6531_ $$9eng$$abiofilm
000015258 6531_ $$9eng$$afood safety
000015258 6531_ $$9eng$$apowder products
000015258 655__ $$ascientifique
000015258 700__ $$aEced-Rodríguez, Laura$$uUniversitat de València, Valencia, Spain
000015258 700__ $$aBeyrer, Michael$$uSchool of Engineering, HES-SO Valais-Wallis, HEI, HES-SO University of Applied Sciences and Arts Western Switzerland
000015258 700__ $$aRodrigo, Dolores$$uDepartamento de Conservación y Calidad de los Alimentos, IATA-CSIC, Paterna, Spain
000015258 700__ $$aRivas, Alejandro$$uDepartamento Tecnología de Alimentos, Instituto Universitario de Ingeniería de Alimentos-FoodUPV, Valencia, Spain
000015258 700__ $$aEsteve, Consuelo$$uUniversitat de València, Valencia, Spain
000015258 700__ $$aConsuelo Pina-Pérez, Maria$$uUniversitat de València, Valencia, Spain
000015258 773__ $$tFoods$$j2024, 13$$k20$$q3251
000015258 8564_ $$uhttps://arodes.hes-so.ch/record/15258/files/Beyrer_2024_sublethal_damage_caused_cold_plasma_bacillus_cereus_cells.pdf$$yPublished version$$9af3624a4-6b82-45da-85dd-bab6fc049a71$$s5721220
000015258 906__ $$aNONE
000015258 909CO $$ooai:hesso.tind.io:15258$$pGLOBAL_SET
000015258 950__ $$aaucun
000015258 980__ $$ascientifique
000015258 981__ $$ascientifique